Oxalate Enzymatic Assay

SKU:
DZ061A

Quantitative in vitro assay for oxalate in human 24‑hour urine to aid in diagnosis and management of kidney stone–related conditions and other metabolic disorders. Product Catalog Number Format Kit DZ061A-K…

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Product Details

Quantitative in vitro assay for oxalate in human 24‑hour urine to aid in diagnosis and management of kidney stone–related conditions and other metabolic disorders.

Product Catalog Number Format
Kit DZ061A-KY1 R1: 1 x 60 mL
R2: 1 x 15 mL
Calibrator DZ061A-CAL Cal: 1 x 5 mL
Control DZ061A-CON Con: 2 x 3 mL
Sample Diluent DZ061A-SD2 SD: 2 x 90 mL

Product Features

  • Quantitative in vitro assay for oxalate in human 24‑hour urine to aid in diagnosis and management of kidney stone–related conditions and other metabolic disorders.
  • Enzymatic method using oxalate oxidase and peroxidase with colorimetric detection at 550 nm, providing high analytical sensitivity (LOQ 0.011 mmol/L; AMR 0.011–4.0 mmol/L).
  • Liquid-stable, ready‑to‑use two‑reagent system (R1 and R2) designed for use on automated chemistry analyzers; sample diluent, calibrator, and controls available separately.
  • Demonstrated precision with total CV typically ≤6.6% across tested urine samples and controls, and excellent correlation (R² = 0.9991) to a predicate method.
  • Minimal interference from common substances at tested levels (ascorbic acid ≤16 mmol/L, hemoglobin, bilirubin, conjugated bilirubin, uric acid), supporting reliable routine use.

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Assay Principle

Oxalates in urine generate carbon dioxide and hydrogen peroxide in the presence of oxalate oxidase7,8. The generated hydrogen peroxide undergoes quantitative oxidative condensation with TOPS and 4-aminoantipyrine through the action of peroxidase (POD). The resulting compound has maximum absorption at 550 nm. The oxalate concentrations in urine samples are proportional to the absorbance values measured.

Intended use

For in vitro quantitative determination of Oxalate in human urine samples. An oxalate test system is a device intended to measure the concentration of oxalate in urine. Measurements of oxalate are used to aid in the diagnosis or treatment of urinary stones or certain other metabolic disorders.

Regulatory Status

FDA 510K Cleared