Diazyme's enzymatic assay for glycated serum protein provides improved specificity and reliability compared to conventional NBT-based methods. The assay is highly precise with a total CV ≤ 1.3%. The assay offers extensive linearity up to 1354 μmol/L.
| Product | Catalog Number | Format | Method |
|---|---|---|---|
| Kit | DZ112B | R1/R2 (Dual Vial Liquid Stable) | Dual Vial Liquid Stable, Enzymatic |
| Calibrator | DZ112B-CAL | Cal: 2 Level (Lyophilized) | |
| Control | DZ112B-CON | Con: 2 Level (Lyophilized) |
Product Features
Diazyme's enzymatic assay for glycated serum protein provides improved specificity and reliability compared to conventional NBT-based methods. The assay is highly precise with a total CV ≤ 1.3%. The assay offers extensive linearity up to 1354 μmol/L. The assay has no significant interference from: ascorbic acid (5 mg /dL); bilirubin (7.5 mg/dL); bilirubin conjugated (5 mg/dL); glucose (2400 mg/dL; hemoglobin (200 mg/dL); triglycerides (2000 mg/dL); and uric acid (35 mg/dL).
Downloads
Informational Articles
- An improved enzymatic assay for glycated serum protein
- A1C Combined With Glycated Albumin Improves Detection of Prediabetes in Africans: The Africans in America Study
- Analytical Evaluation of the Diazyme Glycated Serum Protein Assay on the Siemens ADVIA 1800: Comparison of Results Against HbA1c for Diagnosis and Management of Diabetes
Assay Principle
Diazyme's Glycated Serum Protein Assay uses proteinase K to digest GSP into low molecular weight glycated protein fragments (GPF), and uses Diazyme's specific fructosaminase™, a microorganism originated amadoriase to catalyze the oxidative degradation of Amadori product GPF to yield PF or amino acids, glucosone and H2O2. The H2O2 released is measured by a colorimetric Trinder end-point reaction. The absorbance at 546-600 nm is proportional to the concentration of glycated serum proteins.
Intended use
Diazyme Glycated Serum Protein Assay in conjunction with Diazyme Glycated Serum Protein single calibrator is intended for the quantitative determination of glycated serum proteins (GSP; glycated albumins; fructosamine) in serum. The measurement of glycated serum proteins is useful for monitoring diabetic patients. For in vitro diagnostic use only.
Regulatory Status
