Diazyme's Lipase Assay provides an extended linear range up to 250 U/L with virtually no interferences from triglyceride 300 mg/dL, hemoglobin 150 mg/dL, and bilirubin up to 20 mg/dL. Diazyme's Lipase Assay has excellent inter-assay and intra-assay precision. The assay has an excellent correlation to an existing 6’ Methylresorufin method with an R² of 0.997, y-intercept of 3.9443 and Slope of 0.50054.
The method for the determination of lipase is based on the cleavage of specific chromogenic lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6'methyl-resorufin)-ester emulsified in stabilized micro-particles. In the presence of specific activators of pancreatic lipase as colipase, calcium ions and bile acids, the substrate is converted to 1,2-O-dilauryl-rac-glycerol and glutaric acid-6'-methylresorufinester which decomposes spontaneously to glutaric acid and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample.