Diazyme's Lipase Assay provides an extended linear range up to 250 U/L with virtually no interferences from triglyceride 300 mg/dL, hemoglobin 150 mg/dL, and
bilirubin up to 20 mg/dL. Diazyme's Lipase Assay has excellent inter-assay and intra-assay precision. The assay has an excellent correlation to Beckman™ lipase
reagent with R² = 0.997.
The method for the determination of lipase is based on the cleavage of specific chromogenic
lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6'methyl-resorufin)-ester emulsified
in stabilized micro-particles. In the presence of specific activators of pancreatic lipase as
colipase, calcium ions and bile acids, the substrate is converted to 1,2-O-dilauryl-rac-glycerol
and glutaric acid-6'-methylresorufinester which decomposes spontaneously to glutaric acid
and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation,
is proportional to the activity of lipase in the sample.
The Diazyme Lipase assay in conjunction with lipase assay calibrator set is intended for the in vitro quantitative determination of lipase in serum or plasma.
|Kit (300 Tests)
||R1: 2 x 50 mL
R2: 2 x 11 mL
||1 x 1 mL
||2 x 5 mL