Creatinine Assay (enzymatic)

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Product Features

Diazyme's Creatinine Liquid Reagents Assay offers significant advantages compared to the conventional Jaffe method for Creatinine which is caustic (picric acid)  and can stain analyzer tubing and cuvettes. Diazyme's enzymatic method is non-caustic, non-staining and has no costly hazardous shipping requirements. Unlike  the Jaffe method Diazyme's enzymatic assay shows that the following substances normally present in serum produced less than 10% deviation at the listed  concentrations: Triglyceride at 1000 mg/dL, Ascorbic Acid at 10 mM, Bilirubin at 40 mg/dL, Bilirubin Conjugate at 30 mg/dL, Hemoglobin at 500 mg/dL. The following  substances normally present in urine produced less than 10% deviation at the listed concentrations: Triglycerides at 1000 mg/dL, Ascorbic Acid at 10 mM, Bilirubin  at 40 mg/dL, Bilirubin Conjugate at 40 mg/dL, Hemoglobin at 1000 mg/dL. Packaged for optimal operator convenience, reagent transfer can be eliminated for most  chemistry systems with instrument specific packaging options including Roche™ Hitachi 917 series, Beckman AU (400/600/640/680), Beckman Synchron CX.

Assay Principle

Diazyme's Creatinine Liquid Reagents Assay is a quick, easy to use enzymatic procedure  applicable to routine laboratory instrumentation. Enzymatic methodology is a better clinical  choice for the accurate measurement of creatinine, especially for neonates, pediatrics, and  hematology units. The enzymatic assay for creatinine involves a series of coupled enzymatic  reactions including creatininase enzymatic conversion of creatinine into the product creatine  which itself is converted to sarcosine by creatine amidinohydrolase (creatinase), followed by  oxidation of sarcosine by sarcosine oxidase (SOD) producing hydrogen peroxide. In the presence  of peroxidase (POD) the hydrogen peroxide is quantified at 550 nm by the formation of  a colored dye. Any endogenous creatine present in the sample is removed by creatinase and  sarcosine oxidase during pre-incubation.

Intended use

Diazyme's Creatinine Liquid Reagents Assay, in conjunction with Diazyme's Creatinine Calibrator, is intended for the quantitative determination of creatinine in  serum and urine. Creatinine measurements are used in the diagnosis and treatment of renal diseases, in monitoring renal dialysis, and as a calculation basis for  measuring other urine analytes. For in vitro diagnostic use only.

ProductCatalog NumberFormatMethod
KitDZ072BR1/R2 (Dual Vial Liquid Stable)Dual Vial Liquid Stable,
Enzymatic
CalibratorDZ072B-CalCal: 1 Level 
ControlDZ072B-ConCon: 1 Level 
Regulatory Status