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INTENDED USE:
Diazyme pTau-181 CLIA Kit is for the quantitative determination of Phosphorylated Tau 181 (pTau-181) concentration in EDTA plasma on chemiluminescence analyzer. USA: For Research Use Only. Not for use in diagnostic procedures.
ASSAY PRINCIPLE:
Diazyme pTau-181 CLIA Kit is a sandwich chemiluminescent immunoassay that uses magnetic microbeads coated with an anti- pTau-181 monoclonal antibody for capturing, and Tau-monoclonal antibody labeled with ALP for detection.
Sample or calibrator or control is mixed with the capturing reagent, Anti-Tau monoclonal antibody labeled with AP and the mixture is incubated at 37°C to form a complex. After sedimentation in a magnetic field. The complex is washed to remove unbound proteins and substances. Subsequently, the substrate reagents are added, and a flash chemiluminescent reaction is initiated. The light signal is measured by a photomultiplier as RLU, and the signal is proportional to the concentration of pTau-181 present in the samples.