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INTENDED USE:
Diazyme NfL CLIA Kit is for the quantitative determination of NfL concentration in EDTA plasma on chemiluminescence analyzer. USA: For Research Use Only. Not for use in diagnostic procedures.
ASSAY PRINCIPLE:
Diazyme NfL CLIA Kit is for the quantitative determination of NfL in plasma. Diazyme NfL CLIA Kit is a sandwich chemiluminescent immunoassay that uses magnetic microbeads coated with an anti-NfL monoclonal antibody for capture, and second anti-NfL monoclonal antibody labeled with ALP for detection.
Sample or calibrator or control is mixed with the capture antibody antibody labeled with ALP then the mixture is incubated at 37°C to form a complex. After sedimentation in a magnetic field, the complex washed to remove unbound proteins and substances. Subsequently, the substrate reagents are added, and a flash chemiluminescent reaction is initiated. The light signal is measured by a photomultiplier as RLU, and the signal is proportional to the concentration of NfL present in the samples.