β-Hydroxybutyrate (BHB) Assay

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Background

Beta-hydroxybutyrate (BHB) is a good index for monitoring treatment of diabetic ketoacidosis1-3. In diabetic patients, the measurement of Beta-hydroxybutyrate along with blood glucose is essential for the assessment of the severity of diabetic coma4. It serves as a reliable guide for monitoring the progress of insulin therapy for diabetic ketoacidosis. Additionally, serum Beta-hydroxybutyrate is a key parameter monitored during controlled 24-hour fasts5-7.

Intended Use

The Diazyme β-Hydroxybutyrate (BHB) Assay is for the Quantitative Determination of β-Hydroxybutyrate in human serum or plasma. For in vitro diagnostic use only.

Assay Features
  • Quantitative results
  • Enzymatic method for accurate determination of beta-hydroxybutyrate
  • Lower detection limit (.006 mmol/L) than other commercially available assays
  • Liquid stable reagent, calibrator, and controls requiring no reagent preparation
  • Applications available for chemistry analyzers
Ordering Information
Description Catalog Number Packaging
β-Hydroxybutyrate (BHB) Assay DZ153A-KY1 R1: 1 x 24 mL
R2: 1 x 7 mL
β-Hydroxybutyrate Calibrator DZ153A-CAL CAL: 1 level; (1 x 1 mL liquid)
β-Hydroxybutyrate Controls DZ153A-CON CON: 2 levels; (2 x 3 mL liquid)
BHB Linearity High Level DZ153A-LIN LIN: 1 x 3 mL
Regulatory Status

References
  1. Stephens JM, Sulway MJ, Watkins JP. Relationship of blood acetoacetate and 3-hydroxybutyrate in diabetes. Diabetes 1971;20: 485-9.
  2. Alberti KGMM, Hockaday TDR. Rapid blood ketone body estimation in the diagnosis of diabetic ketoacidosis. Br Med J 1972; ii:565-8.
  3. Harano Y, Kosugi K, Hyosu T, Uno 5, Ichikawa Y, Shigeta Y. Sensitive and simplified method for the differential determina-tion of serum levels of ketone bodies. Clin Chim Acts 1983;134:327-36.
  4. Harano Y, Kosugi K, Hyosu T, Uno S, Ichikawa Y and Shigeta Y, Clin Chem Acta 134, 327 (1983).
  5. Tietz Textbook of Clinical Chemistry. Edited by CA Burtis, ER Ashwood. Philadelphia, WB Saunders Co. 1999
  6. Vassault A, Bonnefont JP, Specola N, et al: Lactate, pyruvate, and ketone bodies. In Techniques in Diagnostic Human Bio-chemical Genetics - A Laboratory Manual. Edited by F Hommes. New York, Wiley-Liss, 1991
  7. Bonnefont JP, Specola NB, Vassault A, et al: The fasting test in paediatrics: application to the diagnosis of pathological hypo- and hyperketotic states. Eur J Pediatr 1990;150:80-85