The Total Bile Acid (NBT Method) Assay is linear up to a concentration of 200 μM and has a correlation coefficient of 0.9918. The following substances normally
present in serum produced less than 10% deviation at the listed concentrations: Triglycerides at 750 mg/dL, Ascorbic acid at 50 mg/dL, Bilirubin at 50 mg/dL and
Hemoglobin at 500 mg/dL.
In the presence of NAD, the enzyme 3- hydroxysteroid dehydro-genase (3- HSD) converts bile
acids to 3-keto steroids and NADH. The NADH formed reacts with nitrotetrazolium blue (NBT)
to form a formazan dye in the presence of diaphorase enzyme. The dye formation is monitored
by measuring absorbance at 540nm and is directly proportional to the bile acids concentration
in the serum sample.
The assay kit is for determination of serum total bile acids (TBA). For Research Use Only in U.S.A.
|Kit (600 Tests)
||R1 (diluent): 1 x 105 mL
R2: 1 x 20 mL
R3: 10 x 10 mL
Cal: 1 x 2 mL vial
||1 x 2 mL
||1 x 1 mL