Enzymatic Homocysteine (3 Reagent)
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MSDS
MSDS - Controls and Callibrators
MSDS - 5-Point Controls and Callibrators
Brochure 		Data Sheet
Product Features
The Diazyme enzymatic homocysteine assay reagent is a liquid stable system, which provides a fast, accurate cost effective alternative to cumbersome and expensive immunochemical methods.  A choice of convenient instrument specific packaging options eliminate reagent transfer for Roche Hitachi, Dade Dimension®, Dade Expand ®, and Beckman Synchron CX, LX and DXC analyzers.  Accurate and reliable, the assay features excellent correlation with HPLC and Immunochemical methods with a linear range of 3-50 µmole/L and intra and inter %CV values of < 5%. The assay has no significant interference from lipemic and hemolytic substances and eliminates the “carry-over” interference with iron or lipase reagents that can occur with other methods. More significantly, the assay is not interfered by the endogenous cystathionine and therefore can be used with confidence in patients with renal disease.   Stable for 14 months from date of manufacture when stored @ 2° - 8° C.
Assay Principle
The Diazyme enzymatic tHcy assay is based on a novel assay principle that assesses the co-substrate conversion product. In the assay, oxidized Hcy is first reduced to free Hcy which then reacts with a co-substrate, SAM, catalyzed by a homocysteine S-methyltransferase. The co-substrate conversion product is amplified by coupled enzymatic cycling reactions. The tHcy level in the sample is indirectly proportional to the amount of NADH conversion to NAD+ (ΔA340nm).
Intended use
The Diazyme Homocysteine Enzymatic Assay kit is intended for the in vitro quantitative determination of total L-homocysteine in serum or plasma. The assay can assist in diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria.
Product Catalog Number Packaging Method/Format
Kit (190 Tests) DZ568A-K R1: 1 x 54 mL
R2: 1 x 11 mL
R3: 1 x 8 mL
 Enzyme Cycling/
Liquid Stable
Calibrator DZ568A-CA5 5 x 3 mL  
Alternate Calibrator DZ568A-CAL 2 x 3 mL  
Control DZ568A-Con 4 x 3 mL