Chloride Ion Assay
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MSDS		 Data Sheet
Product Features
The assay is linear between 70 and 140 mM of chloride in samples. The assay is not interfered by the following substances at the concentrations of: bilirubin up to 200 mg/dL; hemoglobin up to 5000 mg/dL; and no interferences from fluoride and iodide ions. The assay correlates well with the ISE method. Reagents are stable for 12 months from date of manufacture when stored @ 2°-8°C.
Assay Principle
Mammalian α-amylase, which normally involves binding with calcium ion, is deactivated by removing calcium ion by adding a high concentration of EDTA in the absence of chloride anion. The deactivated α-amylase is reactivated by addition of chloride anion, which allows the calcium ion to re-associate with the enzyme. The reactivation of α-amylase activity is proportional to the concentration of chloride anion present. Ethylidene blocked p-nitrophenyl-maltoheptaoside (EPS-G7) is used as the substrate. Reactivated α-amylase hydrolyzes EPS-G7 to Et-Gx and Gy-pNP. Gy-pNP is further hydrolyzed by a coupled enzyme, α-glucosidase to glucose and pNP which is quantitated colorimetrically at 405 nm. The amount of pNP formed is directly proportional to the α-amylase activity in the sample.
Intended use
For the quantitative determination of chloride in serum and plasma samples. For research use only in U.S.A.
Product Catalog Number Packaging Method/Format
Kit (810 Tests) DZ200A-K R1: 4 x 45 mL
R2: 4 x 10 mL
 Colorimetric/Rate
Calibrator DZ200A-Cal 2 x 2 mL  
Control DZ200A-Con 2 x 2 mL