Myeloperoxidase (MPO)
Request product information
Product Features
The linearity of the assay is from 20 – 1300 ng/mL (139 – 9028 pmol/L or 2.0 – 130 mU/mL) in heparin plasma with intra-assay % CV of 1.0 - 3.5% and inter-assay % CV of 1.5 - 4.1%. The following substances normally present in the plasma were tested at levels equal to the following concentrations. Ascorbic acid, bilirubin (free), conjugated bilirubin, triglycerides, naproxen, lovastatin, ibuprofen, salicylic acid, a1-antitrypsin, eosinophil peroxidase (EPO) produced less than 10% deviation when tested up to 0.2 mM, 40 mg/dL, 2.5 mg/dL, 1000 mg/dL, 50 mg/dL, 5.0 mg/dL, 50.0 mg/dL, 60.0 mg/dL, 13.2 mg/dL, and 5 µg/dL, respectively.
Assay Principle
In Diazyme’s MPO enzymatic assay, myeloperoxidase activity is obtained through a two-step reaction by subtracting non- MPO peroxidation from total peroxidation. In the first step, total peroxidation rate A is measured through the reaction of total peroxidases in the sample with hydrogen peroxide, 4-AA and phenol in the reagent; in the second step, an MPO specific inhibitor is added to the reaction mixture to obtain the non-MPO activity. As the result of the two-step reaction, the specific MPO activity is obtained from subtracting the non-MPO activity from the total peroxidase activity, or equals to the reaction rate A (first step) minus the reaction rate B (second step).
Intended use
Diazyme Myeloperoxidase (MPO) Enzymatic Assay it is for the quantitative determination of Myeloperoxidase in plasma samples. For Research Use Only in USA.
Product Catalog Number Packaging Method/Format
Kit (300 Tests)
 DZ178A-K
 R1: 2 x 28 mL
R2: 2 x 14 mL
R3: 2 x 11 mL

Enzymatic/Rate
Liquid Stable
Calibrator DZ178A-Cal 4 x 0.5 mL (2 levels)  
Control DZ178A-Con 6 x 0.5 mL (3 levels)