Triglycerides Assay
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Product Features
The Diazyme Triglyceride assay uses 3 µL sample and takes less than 10 min, and has an excellent correlation with the reference method recommended by the CDC. The reagents are liquid stable with a shelf life of 12 months at 2°- 8°C. The on board stability is 1 month. The assay has a linear range of 0-11.3 mmol/L or 0-1000 mg/dL, and a total CV of < 5.0%.
Assay Principle
The Diazyme Triglyceride assay is based on enzymatic reactions (GPO method) including lipproteinlipase (LPL), glycerol kinase (GK) and glycerol phosphate dehydrogenase (GPO). Triglycerides are hydrolyzed by LPL to liberate glycerol and free fatty acids. Glycerol is converted to glycerol-3-phosphate (G3P) and ADP by GK and ATP. G3P is then converted by GPO to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). H2O2 is quantified by Trinder reaction using 4-aminoantipyrine, 4-chlorophenol and hydrogen peroxidase. The reaction is monitored at 546 nm in a 2 point end manner.
Intended use
The Diazyme Triglyceride assay is intended for the quantitative in vitro determination of total triglycerides in serum or plasma. The assay can assist the assessment of patients suspected of having lipid metabolism disorder such as lipoproteinemia. High blood triglycerides is one of the major risk factors for heart disease as well as for other endocrine disorders.
Product Catalog Number Packaging Method/Format
Kit (1800 Tests) DZ144A-K R1: 4 x 100 mL
R2: 4 x 50 mL		 Colorimetric, endpoint
Liquid Stable
Calibrator DZ144A-CAL 2 x 1 mL  
Control DZ144A-CON 3 x 2 mL