Total Cholesterol Assay
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Product Features
The Diazyme Total Cholesterol assay uses 3 µL sample and takes less than 10 min, and has an excellent correlation with the reference method recommended by the CDC. The reagents are liquid stable with a shelf life of 12 month at 2- 8°C. The on board stability is 1 month. The assay has a linear range of 0-12.9 mmol/L or 0-500 mg/dl, and total CV of < 5.0%.
Assay Principle
The Diazyme Total Cholesterol assay is based on enzymatic reactions (COD method) including cholesterol esterase (CE), cholesterol oxidase (CO) and hydrogen peroxidase (POD). Cholesterol esters are hydrolyzed by CE to produce cholesterol which is then oxidased by CO to produce hydrogen peroxide (H2O2). H2O2 is quantified by Trinder reaction using 4-aminoantipyrine, 4-chlorophenol and POD. The reaction is monitored at 546 nm in a 2 point end manner.
Intended use
Diazyme Total Cholesterol assay reagent is intended for the quantitative in vitro determination of total cholesterol in serum or plasma. The assay can assist the assessment of patients suspected of having lipid metabolism disorder such as lipemia. High blood cholesterol is one of the major risk factors for heart disease.
Product Catalog Number Packaging Method/Format
Kit (300 Tests) DZ143A-K R1: 4 x 100 mL
R2: 4 x 50 mL		 Colorimetric, endpoint
Liquid Stable
Calibrator DZ143A-CAL 2 x 1 mL