| Lipase Assay | |||
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| Product Features The assay provides an extended linear range from 5 to 250 U/L with virtually no interferences from triglyceride (300 mg/dL), hemoglobin (150 mg/dL), and bilirubin up to (20 mg/dL). The Diazyme lipase assay has high precisions with Inter-Run %CV’s of less than 5%, and Intra-Run %CV’s of less than 6% at 215 U/L. The assay has an excellent correlation to Beckman™ lipase reagent with r2 = 0.997. Reagents are stable for 12 months from date of manufacture when stored at 2°-8°C. |
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 Assay PrincipleThe assay is based on the cleavage of a specific chromogenic lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6’methyl-resorufin)-ester emulsified in stabilized micro-particles. The substrate is converted by lipase into 1,2-O-dilauryl-rac-glycerol and glutaric acid-6’-methylresorufin-ester which decomposes spontaneously to glutaric acid and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample. |
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| Intended use The Diazyme Lipase assay in conjunction with lipase assay calibrator set is intended for the in vitro quantitative determination of lipase in serum or plasma. |
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| Product | Catalog Number | Packaging | Method/Format |
| Kit (300 Tests) | DZ132A-K | R1: 2 x 50 mL R2: 2 x 11 mL |
Colorimetric, Liquid Stable |
| Calibrator | DZ132A-Cal | 1 x 1 mL | |
| Control | DZ132A-Con | 2 x 5 mL | |
