Lipase Assay
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 Data Sheet
Product Features
The assay provides an extended linear range up to 250 U/L with virtually no interferences from triglyceride (300 mg/dL), hemoglobin (150 mg/dL), and bilirubin up to (20 mg/dL). The Diazyme Lipase assay has high precision with Inter-Run %CV’s of less than 5%, and Intra-Run %CV’s of less than 6% at 215 U/L. The assay has an excellent correlation to Beckman™ Lipase reagent with r2 = 0.997. Reagents are stable for 12 months from date of manufacture when stored at 2°-8°C.
Assay Principle
The method for the determination of lipase is based on the cleavage of specific chromogenic lipase substrate 1,2-O-dilaurylrac-glycero-3-glutaric acid-(6'methyl-resorufin)-ester emulsified in stabilized micro-particles. In the presence of specific activators of pancreatic lipase as colipase, calcium ions and bile acids, the substrate is converted to 1,2-O-dilauryl-rac-glycerol and glutaric acid-6'-methylresorufinester which decomposes spontaneously to glutaric acid and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample.
Intended use
The Diazyme Lipase assay in conjunction with lipase assay calibrator set is intended for the in vitro quantitative determination of lipase in serum or plasma.
Product Catalog Number Packaging Method/Format
Kit (300 Tests) DZ132A-K R1: 2 x 50 mL
R2: 2 x 11 mL
 Colorimetric,
Liquid Stable
Calibrator DZ132A-Cal 1 x 1 mL  
Control DZ132A-Con 2 x 5 mL