5’-Nucleotidase (5’-NT) Enzymatic Test Kit
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MSDS
Product Features
The assay is linear from 0–300 U/L (r2 >0.99) with intra-assay %CV of less than 4.5% and an inter-assay %CV of less than 6.0%. Assay is specific for 5’-NT and has no detectable reaction with other nucleosides. The assay is not affected by interfering substances such as serum bilirubin up to 40 mg/dL, hemoglobin up to 500 mg/dL, triglycerides up to 1250 mg/dL, ascorbic acid up to 20 mg/dL, and alkaline phosphatase up to 1250 U/L. Stable for 12 months when stored @ 2°-8°C.
Assay Principle
The 5’-NT assay is based on the enzymatic hydrolysis of 5’-monophosphate (5’-IMP) to form inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is then quantified by a Trinder reaction.
Intended use
The 5’-Nucleotidase (5’-NT) assay kit is for the quantitative in vitro determination of 5’-NT activity in human serum samples. Increased enzyme levels in sera are associated with certain forms of liver disease, such as intra- or extra-hepatic obstruction and particularly in cases of hepatic carcinoma and in mastectomy patients with recurrent metastases.
Product Catalog Number Packaging Method/Format
Kit (250 Tests) DZ123A-K R1: 1 x 50 mL
R2: 1 x 25 mL
 Colormetric/Kinetic
Liquid Stable
Calibrator DZ123A-Cal 1 x 1 mL  
Control DZ123A-Con 2 x 1 mL