Carbon Dioxide (CO2)
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MSDS		 Data Sheet
Product Features
The Diazyme enzymatic CO2 assay is a single vial liquid stable reagent system which has a neutral pH, minimizing the effect of CO2 ab­sorption from the air. In addition to a highly stable NADH analog as a co-factor the reagent maintains integrity by rebuilding key reagent components as required for optimal performance.  The reagent provides remarkable full month of calibration curve stability and a 24 month stability claim from date of manufacturer.  The assay is linear up to 50 mmol/L and is highly precise with intra and inter %CV <3.0%. Packaged for optimal operator convenience, reagent transfer can be eliminated for most chemistry systems with instrument specific packaging options including Roche Hitachi 917 series, Olympus AU (400/600/640/680), Beckman Synchron CX, LX and DXC.
Assay Principle
Diazyme Carbon Dioxide Enzymatic Assay is based on two coupled enzyme reactions including phosphoenolpyruvate carboxylase (PEPC) and malate dehydrogenase (MDH). PEPC catalyzes the first reaction which produces oxaloacetate. In the presence of MDH, the reduced cofactor is oxidized by oxaloacetate. This results in a decrease of absorbance at 405 or 415 nm that is directly proportional to CO2 concentration in the sample.
Intended use
Diazyme Carbon Dioxide Enzymatic Assay Kit, in conjunction with Diazyme Carbon Dioxide Calibrator, are intended for the quantitative determination of carbon dioxide (CO2) in serum and plasma. For in vitro diagnostics use only.
Product Catalog Number Packaging Method/Format
Kit (300 Tests) DZ122A-K R1: 5 x 20 mL Colorimetric/Kinetic
Calibrator DZ122A-Cal 1 x 2 mL  
Control DZ122A-Con 2 x 2 mL