Adenosine Deaminase (ADA)
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MSDS
Product Features
The assay is linear from 0-200 U/L (r2 >0.99) and has precisions of Intra assay %CV < 4.5% and Inter assay %CV < 6.0%.The assay is ready-to-use for both manual method and automated chemistry analyzers (Kinetic). The assay is specific for ADA and has no detectable reaction with other nucleosides and is not affected by serum bilirubin up to 20 mg/dL, hemoglobin up to 200 mg/dL, triglycerides up to 750 mg/dL, or ascorbic acid up to 4 mg/dL. The assay is based on H2O2 detection, which is more sensitive and reliable than the method detecting ammonia production and is stable for 12 months when stored @ 2°-8°C shielded from light.
Assay Principle
The ADA assay is based on the enzymatic deamination of adenosine to inosine, which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is then quantified by a Trinder reaction.
Intended use
The Adenosine Deaminase (ADA) assay kit is for the quantitative in vitro determination of ADA activity in human serum or other body fluids. Elevated serum ADA activity has been observed in patients with acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis, liver cirrhosis, viral hepatitis, and hepatoma. Increased ADA activity is also observed in patients with tuberculous effusions. In the USA, the assay is for research use only.
Product Catalog Number Packaging Method/Format
Kit (250 Tests) DZ117A-K R1: 1 x 50 mL
R2: 1 x 25 mL
 Colormetric/Kinetic
Liquid Stable
Calibrator DZ117A-Cal 1 x 1 mL  
Control DZ117A-Con 2 x 1 mL