Sodium (Enzymatic) Assay (Liquid Stable)
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MSDS
Product Features
This method is linear between sodium concentrations of 80 and 180 mmole/L (184 and 414 mg/dL). The following substances normally present in serum produced less than 10% deviation at the listed concentrations: NH4Cl at 1.5 mM, KPi at 2.0 mM, CaCl2 at 7.5 mM, KCl at 10 mM, CuCl2 at 0.5 mM, ZnCl2 at 0.5 mM, FeCl3 at 0.5 mM, Glucose at 5 mM, ascorbate 10 mM, bilirubin at 40 mg/dL, bilirubin conjugate 40 mg/dL, hemoglobin 500 mg/dL, and triglyceride 1000 mg/dL. The assay has good correlation with the ISE method.
Assay Principle
Sodium is determined enzymatically via sodium dependent b- galactosidase activity with ONPG as the substrate. The absorbance at 405 nm of the product O-nitrophenyl is proportional to the sodium concentration.
Intended use
Diazyme Liquid Stable Enzymatic Sodium Assay Kit in conjunction with Diazyme Sodium Low and High Calibrators, are intended for the quantitative in vitro determination of sodium in serum.
Product Catalog Number Packaging Method/Format
Kit (720 Tests) DZ114B-K R1: 4 x 40 mL
R2: 4 x 20 mL
 Enzymatic/Kinetic
Liquid Stable
Calibrator DZ114B-Cal 2 x 3 mL  
Control DZ114B-Con 2 x 3 mL