Potassium (Enzymatic) Assay
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Product Features
The assay shows virtually no affect by the following interfering substances at indicated concentrations: Na+ 150mM, NH4+ 0.5 mM, Ca2+ 7.5 mM, Pi 2.0 mM, ascorbic acid 10.0 mM, Zn2+ 0.5 mM, Fe3+ 0.5 mM, Cu2+ 0.5 mM, triglycerides 1000 mg/dL, hemoglobin 500 mg/dL, conjugated bilirubin 20 mg/dL and unconjugated bilirubin 15 mg/dL. The assay has a linearity range from 2-8 mM. Within precision %CVs are 1.12% and 1.20% for 4.46 mM and 6.86 mM potassium serum samples, respectively. Total precision %CVs are 1.77% and 1.77% for 4.46 mM and 6.86 mM potassium serum samples, respectively. Comparison study showed that the assay has good correlation with existing ISE method with a correlation coefficient of 0.98.
Assay Principle
Potassium is determined spectrophotometrically through a kinetic coupling assay system using potassium dependent pyruvate kinase. Pyruvate generated is converted to lactate accompanying conversion of NADH analog to NAD analog. The corresponding decrease of optical density at 380 nm is proportional to the potassium concentration in the serum.
Intended Use
For in vitro quantitative determination of potassium in fresh human serum. Measurements obtained by this assay are used to monitor electrolyte balance in the diagnosis and treatment of diseases conditions characterized by low or high blood potassium levels. For IVD use only.
Product Catalog Number Packaging Method/Format
Kit (720 Tests) DZ113C-K R1: 4 x 40 mL
R2: 4 x 10 mL		 Enzymatic
Liquid Stable
Calibrator DZ113C-Cal 2 x 3 mL  
Control DZ113C-Con 2 x 3 mL