Enzymatic Fructosamine (Glycated Serum Protein)
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Product Features
The Diazyme enzymatic assay for fructosamine provides improved specificity and reliability compared to conventional NBT-based methods.  The assay is highly precise with a mean intra assay <2% and mean inter-assay CV of <3%.  The assay offers extensive linearity from 30 mmol/L to 1200 mmol/L.  The assay has no significant interference from: ascorbic acid (4 mg/dL); bilirubin (2 mg/dL); glucose (1200 mg/dL; hemoglobin (100 mg/dL); triglycerides (250 mg/dL); and uric acid (15 mg/dL). The reagent is stable for 12 months when stored @ 2°-8°C
Assay Principle
The Diazyme enzymatic assay for glycated serum proteins (GSP) uses a proteinase to digest GSP into low molecular weight glycated protein fragments (GPF), and uses Diazyme’s specific Fructosyl Amine-Oxygen Oxidoreductase to catalyze the oxidative degradation of Amadori product of GPF to yield amino acids, glucosone and H2O2. The H2O2 released is measured by a colorimetric Trinder end-point reaction. The absorbance at 600 nm is proportional to the concentration of glycated serum proteins (GSP).
Intended use
The assay kit is for the quantitative in vitro determination of Glycated serum proteins (enzymatic fructosamine) in human serum. Glycated serum protein is formed due to a non-enzymatic Maillard reaction between glucose and amino acid residues of proteins. In diabetic patients, elevated blood glucose levels correlate with increased glycated serum protein formation. Glycated serum protein is a medium term indicator of diabetic control (2-3 weeks).
Product Catalog Number Packaging Method/Format
Kit (180 Tests)
 DZ112A-K
 R1: 2 x 20 mL
R2: 2 x 5 mL
 Colorimetric, endpoint
Powder
Calibrator DZ112A-Cal 2 x 1 mL  
Control DZ112A-Con 2 x 1 mL