α-L-Fucosidase (AFU)

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 Data Sheet
Product Features
The Diazyme AFU assay is a single reagent, one step method that has a linear range of 0-300 U/L. The reagent is ready-to-use for both manual and automatic chemistry analyzers. The assay is specific for AFU and has no detectable reaction with other glycosidases. The assay is not affected by serum bilirubin up to 100 mg/dL, hemoglobin up to 200 mg/dL, triglycerides up to 750 mg/dL, or ascorbic acid up to 4.4 mg/dL. The assay has precisions of Intra %CV < 5.1% and Inter CV% <6.2% and is stable for 24 months from the date of manufacture when stored @ 2°-8°C shielded from light.
Assay Principle
The AFU assay is based on the enzymatic cleavage of the synthetic substrate 2-chloro-4-nitrophenyl-α-L-fucopyranoside to α-L-fucoside and 2-chloro-4-nitrophenol, which is kinetically quantified by measuring the absorbance at 405 nm.
Intended use
The α-L-fucosidase (AFU) assay kit is for the quantitative in vitro determination of AFU activity in serum samples. For research use only in U.S.A.
Product Catalog Number Packaging Method/Format
Kit (200 Tests) DZ082B-K R1: 2 x 25 mL Colormetric/Kinetic
Liquid Stable
Calibrator DZ082B-Cal 1 x 1 mL  
Control DZ082B-Con