Creatinine Assay (enzymatic)
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MSDS
Product Features
Diazyme’s enzymatic creatinine reagent offers significantly advantages compared to the conventional Jaffe method for creatinine which is caustic (picric acid) and can stain analyzer tubing and cuvettes. Diazyme enzymatic method is non-caustic, non-staining and has no costly hazardous shipping requirements. Unlike the Jaffe method Diazyme’s enzymatic assay shows virtually no interference from ascorbic acid (50 mg/dL), bilirubin (16mg/dL), hemoglobin (200 mg/dL) and triglycerol (1000 mg/L). Packaged for optimal operator convenience, reagent transfer can be eliminated for most chemistry systems with instrument specific packaging options including Roche Hitachi 917 series, Olympus AU (400/600/640/680), Beckman Synchron CX, LX and DXC.  Reagents are stable for 18 months when stored at 2°-8°C.
Assay Principle
The enzymatic assay involves a series of coupled enzymatic reactions including conversion of creatinine into creatine, and the product creatine is converted to sarcosine by creatine amidinohydrolase (Creatinase), followed by the oxidation of sarcosine by sarcosine oxidase (SOD) producing hydrogen peroxide (H2O2) which is quantified by a Trinder reaction.
Intended use
The assay kit is for the quantitative in vitro determination of creatinine in human serum or urine. Determination of serum or urinary creatinine is a useful diagnostic tool for kidney diseases such as acute and chronic nephritis and other disorders such as urethrophaxis, mercurialism and nephrosis.
Product Catalog Number Packaging Method/Format
Kit (200 Tests) DZ072B-K R1: 1 x 60 mL
R2: 1 x 20 mL
 Colorimetric, endpoint
Liquid Stable
Calibrator DZ072B-Cal 1 x 1 mL  
Control DZ072B-Con 1 x 1 mL