N-Acetyl-ß-D-glucosaminidase (NAG)
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MSDS		 Data Sheet
Product Features
The assay is ready-to-use for manual methods and adaptable for analyzers. The assay is specific for NAG and has no detectable reaction with other glycosidases and has excellent sensitivity with a linear range from up to 200 U/L and excellent precision (CV <5%) . There is no significant interference from hemoglobin or albumin. Interference from bilirubin occurs only at levels higher than 5 mg/dL. The reagent is stable for 12 months from date of manufacture when stored at @ 2°-8°C.
Assay Principle
The Diazyme NAG assay uses a specific substrate, 2-methoxy-4-(2’nitrovinyl)-phenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (MNP-GlcNAc) that is hydrolyzed by NAG to produce 2-methoxy-4-(2’-nitrovinyl)-phenol product. The product formation is detected at 505 nm upon addition of an alkaline buffer.
Intended use
The N-acetyl-β-D-glucosaminidase (NAG) assay kit is for determination of NAG in urine samples. For Research Use Only in the USA.
Product Catalog Number Packaging Method/Format
Kit (540 Tests) DZ062A-K R1: 1 x 75 mL
R2: 1 x 15 mL
R3: 1 x 30 mL
 Colorimetric, endpoint
Liquid Stable
Calibrator DZ062A-Cal 1 x 2 mL  
Control DZ062A-Con 2 x 2 mL