Diazyme's Direct Bilirubin Assay is a dual stable liquid method that provides an enhanced level of accuracy, reliability and freedom from interference
compared to conventional Diazo procedures. The test offers an extended testing range of 0.1 to 20 mg/dL, provides excellent precision with
Intra-Assay Precision of CV% of <3%, Inter-Assay Precision CV% of <4%. The assay demonstrates no significant interference from ascorbate acid up to 50 mg/dL
and hemoglobin up to at least 500 mg/dL. With a unique combination of low sample size requirements and interference free testing Diazyme Direct Bilirubin Assay
is an ideal choice.
When a sample is mixed with the reagent containing the vanadate, at around pH 3, direct
bilirubin in the sample is oxidized to biliverdin. This causes the absorbance of yellow, specific
to bilirubin, to decrease. Therefore, the direct bilirubin concentration in the sample can be
obtained by measuring the absorbance before and after the vanadate oxidation.
The Diazyme Direct Bilirubin Assay is an in vitro colorimetric assay for the quantitative determination of direct bilirubin in serum. Determination of serum bilirubin
is useful in the screening of liver function disorders or in the diagnosis of jaundice.
|Kit (900 Tests)
||R1: 4 x 70 mL
R2: 4 x 18 mL
||1 x 3 mL
||2 x 5 mL