GlycoMark (1,5-AG) reflects hyperglycemia above the renal threshold over the preceding 1-2 weeks which is complimentary to HbA1c and Glycated Serum Protein determinations. The test provides a useful, unique perspective of patient’s recent hyperglycemic excursions, which may not be evident from standard glycemic markers. Hyperglycemic excursions may be associated with glycemic variability, which can be investigated through more frequent self-monitoring of blood glucose (SMBG) or continuous glucose monitoring (CGM).
GLYCOMARK test is an enzymatic method consisting of a two-reagent test kit (Reagent 1 and Reagent 2). The test has been developed to be used with a fully automated chemistry analyzer. The test system also includes the 1,5-AG calibration standard solution (50 µg/mL), and a two-level control set (“Low “ and “High”). The calibration standard solution and control set are purchased separately. The method uses the enzyme pyranose oxidase (PROD) as an oxidase of 1,5-AG to oxidize the 2nd position hydroxyl group of 1,5-AG and to detect the generated hydrogen peroxide by colorimetry using peroxidase (POD). As PROD also reacts with glucose, the sample is pretreated by enzyme reaction using glucokinase (GK). Glucose is converted into glucose-6-phosphate (G-6-P), a species non-reactive with PROD. To drive the reaction to completion, an adenosine triphosphate (ATP)-regenerating system consisting of pyruvate kinase (PK) and Phosphoenol pyruvate (PEP) is utilized. As ATP is converted to adinose diphosphate (ADPP), PK, in the presence of PEP, catalyzes the phosphorylation of ADP back to ATP. Following the conversion of glucose to G-6-P, the assay is rendered specific for 1,5-AG.
The GLYCOMARK test provides quantitative measurement of 1,5-anhydroglucitol (1,5-AG) in serum or plasma. The test is for professional use, and is indicated for the intermediate term monitoring of glycemic control in people with diabetes.
||R1: 1 x 20 mL
R2: 1 x 10 mL
||3 x 5 mL
||Low: 3 x 2 mL
High: 3 x 2 mL